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▲Date時間:2025/04/01 (Tue. 二)
10:30~12:00:D603儀器介紹簡報Introduce
13:30~15:30:D921儀器上機示範Operation
(中午休息 Lunch break )
▲Location地點:慈濟大學勤耕樓D603貴儀中心及D921
人數限定10人 up to 10 person
主辦單位:研發處貴重暨共同儀器中心 分機11409
協辦單位:騰達行企業(講者:林宇慶博士)
報名辦法:慈濟大學新校務系統活動報名 (單號11400196)
▲八通道高階分子交互作用即時分析系統ForteBio Octet RED96e System
利用生物膜干涉技術 BLI (Biolayer Interferometry) 原理,用以偵測分子間交互作用,應用於測定分子之間結合與解離相關常數 (KD、 kon 與 koff)。可直接使用於分子交互作用試驗,與樣品接觸之感測元件為耗材式設計,防止樣品之間交叉污染,因此檢測結束後,樣品可以回收利用。8 通道設計可選擇單次平行上樣 1~8 個樣品進行測試,且樣品之間完全獨立不干擾,增加實驗彈性以進行高通量交互作用實驗。本系統可應用於新藥開發、抗體篩選、結合特異性分析、蛋白質定量分析、分子鑑別、酵素反應、細胞信號傳遞與蛋白質體學等有關生物分子間之交互作用。
The Octet® RED96e system detects a diverse range of biomolecules from small molecules to proteins to mammalian cells. The Octet platform offers an advanced fluidics-free approach with a wide variety of off-the-shelf Dip and Read biosensors for rapid binding kinetics and quantitation analysis.
The system utilizes ForteBio’s Bio-Layer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like cell culture supernatants and lysates.
The 8-channel Octet RED96e system performs quantitation of 96 samples in 32 minutes, and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling more flexibility in sample throughput when needs change.
▲應用研究領域
1. 新藥開發 Drug Discovery
2. 抗體篩選 Antibody Screening
3. 配體垂釣 Ligand Fishing
4. 結合特異性 Binding Specificity
5. 蛋白質定量 Protein Quantization
6. 分子鑑別 Molecular Recognition
7. 細胞信號傳導 Cell Signal Transduction
8. 蛋白質體學 Proteomics
9. 疫苗開發 Vaccine Development
請上校務系統報名,敬邀踴躍參與。
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