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||【名稱】：1080115【Lecture】Li-Foong Yoong, PhD. (Research Scientist, Laboratory for Neurodiversity, RIKEN Center for Brain Science JAPAN)
Time: 09:00-10:00 Tuesday, January 15, 2019
Venue: Conference Room 2
Li-Foong Yoong, PhD.
Research Scientist, Laboratory for Neurodiversity, RIKEN Center for Brain Science JAPAN
How do neurons build their complex dendrite arbors?
Dendrites are the crucial structure of neurons where signals are received and propagated. Neurons require highly branched dendritic arbor morphologies to wire together neural networks and define the individual functional properties of the cell. The intricate cell biological mechanisms by which such complex dendritic arbor architectures are established remain largely unknown. Importantly these mechanisms are often disrupted in neurological disorders and may be utilized during neuronal regeneration. To understand how final morphology is achieved, assaying not just the final dendrite shape but rather the continuous imaging of neurons during dendrite outgrowth and arborization is required. However, live imaging of mammalian neuron has been proven challenging as they take weeks and sometime months to develop. On the other hand, insect develops and matures its nervous system in just days. Taking advantage of this system, our lab uses Drosophila melanogaster as a model for deciphering the molecular mechanisms underlying the complex dendrite arborization processes (Nature Neuroscience. 2015;18(10):1437-45, Nature Neuroscience. 2011;15(2):224-33, Neuron. 2007;56(6):963-78.)
In this talk, I will discuss our recent work of studying complex dendrite formation using a novel non-invasive, in vivo time-lapse microscopy approach. We performed live imaging-based RNAi screening coupled with computer vision-based quantification, we identify the atypical myosin (MyoVI) as a principal player in dendrite growth cone remodeling process. We show that MyoVI regulates filopodia actin dynamic which in turns govern microtubule invasion leading to growth cone splitting to generate major branches. Overall, our findings establish the critical early individual cell biological events feed-forward to subdivide the mature dendrite arbor, and which are differentially utilized by different neuron classes to create diversity in neuron form and function. This process defines essential neuronal features underlying circuit wiring and computation.
Organizer: Institute of Medical Sciences
【參加對象】：慈大教師 慈大職員 慈大學生 校外人士